Investigation of relationship between hyper-homocysteinemia and experiment au-toimmune encephalomyelitis mice
10.3969/j.issn.1000-484X.2014.12.025
- VernacularTitle:高同型半胱氨酸血症对实验性变态反应性脑脊髓炎小鼠的影响
- Author:
Xiang ZHANG
;
Jian QIAO
- Publication Type:Journal Article
- Keywords:
Hyper-homocysteinemia;
Multiple sclerosis;
Experiment autoimmune encephalomyelitis
- From:
Chinese Journal of Immunology
2014;(12):1697-1700,1704
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between hyper-homocysteinemia ( HHcy) and experiment autoimmune en-cephalomyelitis (EAE) mice,and to study the cause and effect of HHcy in multiple sclerosis (MS).Methods: Four groups were included in this study:healthy C57BL/6 mice and EAE mice fed with chow(HC and EAE group),healthy mice and EAE mice supple-mented with 4%(w/v) methionine in the drinking water (HC+Hcy and EAE+Hcy group).Clinical symptoms,the levels of Hcy and cytokines ( TNF-α,IFN-γand IL-17) were evaluated at different time points.Results:EAE group had no greater Hcy than HC group ( P>0.05 ).The clinical scores and weight loss of mice from EAE+Hcy group were more severe than those from EAE group during the course of EAE ( P<0.05 ).EAE+Hcy group had higher levels of TNF-αand IFN-γthan EAE group ( P<0.05 ) ,while IL-17 was about the same.Conclusion:Mice do not have gradually increased Hcy during the course of EAE.HHcy worsen the symptoms of EAE by pro-moting the production of TNF-αand IFN-γ,but not IL-17.
