The curative effect of targeted siRNA of vascular endothelial growth factor combined endostatin on collagen-induced arthritis rat
10.3760/cma.j.issn.1007-7480.2014.08.010
- VernacularTitle:内皮抑素联合靶向血管内皮生长因子的siRNA对大鼠胶原诱导性关节炎疗效的研究
- Author:
Ye LI
;
Xuefei XIA
;
Yanrong FAN
- Publication Type:Journal Article
- Keywords:
Arthritis,experimental;
Endostatins;
RNA,small interfering;
Vascular endothelial growth factors
- From:
Chinese Journal of Rheumatology
2014;18(8):545-548,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of treatment with endostatin combined with siRNA targeting VEGF on rats with collagen-induced arthritis (CIA).Methods Two mg/ml bovinetype Ⅱ collagen was injected into the rat footpad to build up the animal model of CIA.The experimental animal models were treated with endostatin combined with siRNA targeting VEGF 18 days later after immunization and the treatment ended 32 day later.The efficacy was evaluated by the weight,foot and ankle volume of rats.The levels of VEGF in plasma were detected by enzyme-linked immunosorbent assay (ELISA).The VEGF distribution within synovial tissue was detected and examined by immunohistochemical technique.The pathological changes of CIA were evaluated by the pathological changes of the biopsied ankle joints.Student's t test was used to evaluate the experimental data.Results The ELISA test showed that comparing with the model group (17.5±0.3),the endostatin group (15.7±0.3) ng/L and the endostatin combined siRNA targeting VEGF group (14.7±0.5) ng/L showed a significant efficacy in the treatment of CIA in rats (P＜0.05).The endostatin group (135±27) and the endostatin combined siRNA targeting VEGF group (126±71) were different in the number of VEGF in plasma and the VEGF distribution within synovial tissue (P＜0.05),the symptoms of arthritis in these rats were reduced than the model group.Conclusion Endostatin combined siRNA targeting VEGF has good therapeutic effect on rats with CIA.
