Genomic characterization of human papillomavirus type 16 from Anhui province
- VernacularTitle:安徽省人乳头瘤病毒16型全基因组的结构特征
- Author:
Yunfeng SUN
;
Tong JIANG
;
Chuanjun CHEN
- Publication Type:Journal Article
- Keywords:
human papillomavirus type 16;
genome;
clone;
sequence analysis
- From:
Acta Universitatis Medicinalis Anhui
2014;(10):1456-1459,1460
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone human papillomavirus type 16 genome from Anhui province, analyze genome se-quence of HPV16 and study its genomic characteristics. Methods Five pathological specimens of cervical cancer from Anhui province were collected and the total DNA was extracted. Specific primers were designed to clone HPV16 genome in four fragments. The sequence of four fragments was assembled manually and nucleotide sequence was analyzed after sequencing. Results A cervical pathological sample containing HPV16 was detected and the genome sequence with full length of 7 906 nts (GenBank accession number:KC935953) was obtained. Sequence alignment of genome nucleotide sequence showed that HPV16 of Thailand and HPV16 of Japan were more similar to HPV16 of Anhui (HPV16-Anhui) than other HPV genome nucleotide sequence, their similarity reached 99. 5%. Phylogeny tree analysis demonstrated that HPV16-Anhui and other 7 HPV16 clustered into a single branch. Con-clusion HPV16 genome nucleotide sequence is obtained from Anhui province for first time with great significance for further understanding of HPV16 variation from Anhui even east China.
