Cardioprotective effect of RISK signaling pathway on diazoxide postconditioning in isolated ischemic and reperfused rat hearts
10.3969/j.issn.1001-1978.2014.09.016
- VernacularTitle:RISK途径在二氮嗪后处理离体大鼠心肌中的保护作用
- Author:
Ying WANG
;
Ping XIE
;
Lin ZHANG
;
Xingkui LIU
;
Tian YU
- Publication Type:Journal Article
- Keywords:
diazoxide;
postconditioning;
ischemica reperfusion injury;
RISK;
cardioprotection;
mitochondri-al ATP sensitive potassium channel
- From:
Chinese Pharmacological Bulletin
2014;(9):1257-1261,1262
- CountryChina
- Language:Chinese
-
Abstract:
Aim To discuss whether specific mitochon-drial ATP-sensitive potassium channel opener diazoxide ( DZ ) postconditioning activates RISK signaling path-way to protect isolated rat hearts against ischemica reperfusion injury ( IRI ) . Methods Langendorff de-vice was used to establish rat in vitro model of myocar-dial ischemia reperfusion. SD rats were randomly di-vided into normal group ( NOR ) , control group ( CON ) , diazoxide after treatment group ( DZ ) , and LY294002 antagonistic nitrogen Triazine group ( DZ +LY) , with 8 cases in each. The following was com-pared:①whether heart function of each group changed at the end of equilibration and reperfusion; ② at the end of myocardial perfusion and separation, protein was extracted, and protein kinase B ( PKB / Akt ) , P70S6 kinase (P70S6K), endothelial nitric oxide syn-thase ( eNOS) phosphorylation level of expression were analysed by Western blot. Results ① Indicators of changes in heart function: for DZ group at the end of reperfusion , HR , CF , LVDP , LVEDP , +d p/d tmax and -dp/dtmax were significantly better than those in CON group and DZ + LY group ( P <0.01 ) , but worse than those in NOR group ( P <0.01 ); there was no statistical difference in cardioac function at the end of equilibration. ② For DZ group at the end of reperfu-sion Akt, P70S6K, eNOS phosphorylation level of ex-pression were significantly higher than those in NOR group, CON group, and DZ + LY group (P<0.01). There was no difference in expression level of ERK1/2 phosphorylation ( P >0.05 ) . Conclusion Diazoxide postconditioning through the activation of RISK signa-ling pathway can protect isolated rat hearts against is-chemia reperfusion injury.
