Screening of the nucleotide sequences affected by α-synuclein in vitro
10.3969/j.issn.1001-1978.2014.09.010
- VernacularTitle:α突触核蛋白作用核苷酸序列的体外筛选研究
- Author:
Kaili MA
;
Liankun SONG
;
Yuhe YUAN
;
Ying ZHANG
;
Li LI
;
Jinling YANG
;
Ping ZHU
;
Naihong CHEN
- Publication Type:Journal Article
- Keywords:
α-synuclein;
nucleotide sequences;
circu-lar dichroism;
GC-box like sequence;
nuclear localiza-tion;
Parkinson's disease
- From:
Chinese Pharmacological Bulletin
2014;(9):1225-1228,1229
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the screening of the nucleo-tide sequences might be affected by α-syn in vitro. Methods The nucleotide sequences were synthesized according to the feature of base composition, and then mixed with the α-syn-GFP. The CD was used to ana-lyse the changes of the peak. Result The peak of the CD changed greatly when the α-syn-GFP mixed with the GC-box like sequence. Conclusion The α-syn-GFP might affect the GC-box like sequence after trans-located into the nuclei. Then, it plays a role in physio-logical and pathological conditions by affecting the reg-ulation of gene expression.
