Study of mechanism of DXM and NAC inhibiting expression of IL-8 and ICAM-1 in A549 cells
10.3969/j.issn.1001-1978.2014.09.023
- VernacularTitle:地塞米松及N-乙酰半胱氨酸抑制A549细胞IL-8及ICAM-1表达的机制研究
- Author:
Qi XIANG
;
Xin FU
;
Pixin RAN
;
Jin ZHANG
;
Xiwei ZHENG
;
Juan CHEN
;
Yuanyuan GUO
- Publication Type:Journal Article
- Keywords:
pulmonary disease;
chronic obstructive;
the inflammatory response;
dexamethasone;
N-acetyl cyste-ine;
acetylation signal
- From:
Chinese Pharmacological Bulletin
2014;(9):1293-1297,1298
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the mechanism of DXM and NAC inhibiting the expression of IL-8 and ICAM-1 in A549 cells. Methods The expression of IL-8 and ICAM-1 was detected by ELISA and flow cytometry re-spectively; the expression of GR,HDAC,AP-1,NF-κB was detected by Western blot, while the activity of HDAC was detected by spectrophotometry. ResultsThe increasing expression of IL-8 and ICAM-1 induced by TNF-α could be inhibited by DXM and NAC in A549 cells. DXM could inhibit the transcribed activa-tion of AP-1,NF-κB, and the expression of HDAC and its activity induced by TNF-α and LPS; NAC only in-hibited the transcribed activation of NF-κB, while it had no affection on the transcribed activation of AP-1 and the expression of HDAC and its activity. Conclu-sions DXM and NAC both have the anti-inflammatory effect. DXM plays the role of anti-inflammation through increasing the expression and activation of HDAC, in-hibiting the transcribed activation of AP-1 and NF-κB, while NAC has no effect on the expression and activa-tion of HDAC, which shows that NAC does not exert anti-inflammatory effect through acetylation signal.
