Inhibition effect of Guizhi decoction on cardiac sympathetic sprouting
10.3969/j.issn.1001-1978.2014.09.029
- VernacularTitle:桂枝汤对4-甲基邻苯二酚诱导心脏交感神经芽生的抑制作用
- Author:
Xiao LI
;
Ping JIANG
;
Haiqing LIN
;
Xiangdong XU
;
Jinlong YANG
;
Dufang MA
;
Xiaohua YU
;
Yuehua JIANG
- Publication Type:Journal Article
- Keywords:
cardiac sympathetic sprouting;
cardiac autonomic nerve remodeling;
4-methylcatechol;
meto-prolol;
Guizhi decoction;
heart
- From:
Chinese Pharmacological Bulletin
2014;(9):1320-1324,1325
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the preventive effect of Guizhi decoction on cardiac sympathetic sprouting induced by 4-Methylcatechol (4-MC) . Methods The rat models of cardiac sympathetic sprouting were in-duced by 10 mg · L-1 4-MC ( 10μg · kg-1 body weight, i. p. ) . Guizhi decoctions ( with different pro-portion between Ramulus Cinnamomi and Radix Paeon-iae Alba, 1 : 1, 1 : 2 and 2 : 1) and metoprolol were administered to the rats. Heart rate and electrocardio-gram ( ECG ) were observed, the content of norepi-nephrine (NE), growth associated protein (GAP-43), tyrosine hydroxylase ( TH ) and acetylcholine transfer-ase enzyme ( CHAT) in myocardial homogenate of left ventricular and right atrial were determined by ELISA method, and immunofluorescence assay was used to observe cardiac nerve sprouting and sympathetic distri-bution. Results 4-MC caused cardiac sympathetic sprouting and parasympathetic was not influenced. Heart rate of the model group was improved significant-ly and higher than that of the other groups . Compared with the model group, the content of NE, GAP-43 and TH in left ventricle and right atrium of the metoprolol group and Guizhi decoction group were decreased( P<0.05 ) , and the immunofluorescence result showed that the distribution of TH positive nerve was reduced sig-nificantly(P<0.05). It was demonstrated that Guizhi decoction of the proportion between Ramulus Cinnamo-mi and Radix Paeoniae Alba 1 : 1 and 1 : 2 had the best efficacy, which was similar to the efficacy of meto-prolol. Conclusion Guizhi decoction ( with the pro-portion between Ramulus Cinnamomi and Radix Paeon-iae Alba 1 : 1 and 1 : 2 ) effectively inhibits the cardi-ac sympathetic sprouting induced by 4-MC.
