Establishment and application of a molecular typing system of Staphylococcus aureus based on resolution melting
10.3969/j.issn.1673-4130.2014.17.038
- VernacularTitle:金黄色葡萄球菌熔解曲线分子分型体系的建立及应用
- Author:
Bing WANG
;
Yinghui LI
;
Qinghua HU
;
Yiman LIN
;
Xiaolu SHI
;
Yaqun QIU
;
Lianhua HE
;
Miaoling CHEN
;
Pingfang WU
;
Shule XU
- Publication Type:Journal Article
- Keywords:
Staphylococcus aureus;
resolution melting;
molecular typing
- From:
International Journal of Laboratory Medicine
2014;(17):2354-2355,2358
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a molecular typing system of Staphylococcus aureus by using resolution melting for food-poi-soning fast tracing.Methods Primers were designed and synthesized according to the literature of VNTR in Staphylococcus au-reus ,and were used to perform molecular typing on the strains which had detected by PFGE,then 4 types of VNTRs were with higher discriminatory power were selected.On this basis,we established a molecular typing system for the detection of 59 Staphy-lococcus aureus isolated from food poisoning.Results The molecular typing system has good precision for detection.The standard deviation(s)of within-batch repetitive experiments were 0.03 -0.05 ℃,between-batch repetitive experiments were 0.04 -0.06℃,between-day repetitive experiments were 0.04-0.06 ℃.At the same time,the 59 strains of Staphylococcus aureus were divided into 19 types which were 11 epidemic clones and 8 sporadic clones.The correlation coefficient of Simpson was 0.916 4.Conclusion The molecular typing system for Staphylococcus aureus based on resolution melting was simple,fast and repeatable.It can be ap-plied to fast tracing and screen of Staphylococcus aureus in food poisoning.
