Determination of Cyasterone in Shenshixiao Capsules by HPLC
- VernacularTitle:HPLC法测定肾石消胶囊中杯苋甾酮的含量
- Author:
Yingxia GUO
;
Dongliang HE
;
Jingli SONG
;
Juan LI
- Publication Type:Journal Article
- Keywords:
Cyasterone;
HPLC;
Shenshixiao capsules
- From:
China Pharmacist
2014;(9):1594-1595,1596
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for the determination of cyasterone in Shenshixiao capsules. Methods: Using an HPLC method, the column was an Agilent C18 (250 mm × 4. 6 mm,5 μm) column, the mobile phase was methanol-water with gradient elution. The detection wavelength was 243nm, the flow rate was 1. 0 ml·min-1 ,and the temperature of column was room temperature. Results:The linear relationship of cyasterone was good within the concentration range of 0. 025-0. 247 μg(r=0. 999 6), the average recovery was 99. 4%, and RSD was 0. 76%. Conclusion:The method is rapid, simple and accurate, and can be used in the quality control of the preparation.
