Effects of glucose concentration on G6PD activity and respiratory burst of normal human′s neutrophils in vitro
10.3969/j.issn.1671-8348.2014.24.022
- VernacularTitle:不同葡萄糖培养浓度对健康成人外周血白细胞G6PD活性及呼吸爆发功能的影响
- Author:
Huiyan ZENG
;
Ying CAO
;
Yaoming XUE
- Publication Type:Journal Article
- Keywords:
neutrophils;
respiratory burst;
G6PD
- From:
Chongqing Medicine
2014;(24):3182-3183,3186
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effects of glucose concentration on G 6PD activity and respiratory burst of normal hu-man′s neutrophils in vitro .Methods Normal human′s neutrophils were cultured in different glucose concentration for 8 hours ,as-sayed G6PD activity by spectrophotometric method and determining ROS content by fluorescent probe DCFH-DA .Results G6PD activity and ROS of 15 mmol/L group and 25 mmol/L group were significant lower than before ,when the 5 mmol/L group and L-GLU group didn′t have significant change with time goes by .And G6PD activity and ROS of 25 mmol/L group were the lowest in all groups(P<0 .01) .Conclusion High glucose may induce G6PD activity decreased and cause respiratory burst dysfunction as a stimulating factor .The stimulation intensity was increased with the increase of glucose concentration .It′s the probable mechanism on susceptibility to infections in patients with diabetes mediated by dysfunction of respiratory burst in leucocyte .
