Study on genotypes and phenotypes of metallo-β-lactamase in multidrug-resistant Pseudomonas aeruginosa
10.3969/j.issn.1673-4130.2014.15.009
- VernacularTitle:多重耐药铜绿假单胞菌的金属酶表型与基因型研究
- Author:
Xiaoying YANG
;
Hongqiu WU
;
Qizhi XIAO
;
Changzheng YAN
;
Donghai YANG
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
polymerase chain reaction;
genotype
- From:
International Journal of Laboratory Medicine
2014;(15):1989-1990,1992
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the generation of metallo-β-lactamase(MBLs) and its related gene carrying situation in the clini-cal isolates of Pseudomonas aeruginosa .Methods Ceftazidime and imipenem were adopted to preliminarily screen MBLs of Pseudo-monas aeruginosa .The phenotypic confirmatory of imipenem-resistant and ceftazidime-resistant Pseudomonas aeruginosa was per-formed by using 2-mercaptopropionic acid (2-MPA) or EDTA synergy test and the MBLs genotypes of the positive strains in the preliminary screen were detected by PCR .Results The positive rate of the MBLs preliminary screen test in multi-resistant strains was 10 .9% ,and the positive rate of the MBLs in multi-resistant strains detected by CAZ/EDTA ,CAZ/2-MPA ,IMP/EDTA and IMP/2-MPA was 7 .5% ,7 .9% ,8 .8% and 9 .5% respectively .The positive rates of ipm1 and vim gene by PCR were 10 .4% and 8 .3% respectively .The strains with positive spm ,sim1 and gim were not found .Conclusion The MBLs test results detected by different methods are different ;MBLs genes carying ipm1 and vim are the main reason for carbapenem-resistant multi-drug resist-ant Pseudomonas aeruginosa in the hospital .
