The allogeneic bone marrow mesenchymal stem cells improve rats cutaneous wound healing
10.3760/cma.j.issn.1008-6706.2014.19.006
- VernacularTitle:同种异体间充质干细胞改善大鼠皮肤愈合的研究
- Author:
Yu CAO
;
Li ZHAO
;
Weisen JIN
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Wound healing;
Growth factor;
Western blot
- From:
Chinese Journal of Primary Medicine and Pharmacy
2014;(19):2898-2901,2902
- CountryChina
- Language:Chinese
-
Abstract:
Objective This experiment used the allogeneic bone marrow mesenchymal stem cells to cure the wound in rats .And to observe the effect and safety of bone marrow mesenchymal stem cells injection for wound heal -ing.Methods The rats with wound were divided into two groups .The rats bone marrow mesenchymal stem cells were isolated,cultured,purified,amplified and passaged.Flow cytometry instrument was used to identify the immune pheno-type of rats bone marrow mesenchymal stem cells .Local injected the rats-bone marrow mesenchymal stem cells in the rats.The difference of wound healing rate between the experimental group and control group were statistically ana -lyzed.Western blot was used to detect the expression of keratinocyte growth factor (KGF) in two groups.Histopatho-logical change of the wound was observed .Results The wound healing rate between the two groups had significantly statistical difference.After 3 days,7 days,14 days,the wound healing rates of the experimental group were (21.95 ± 8.11)%,(56.25 ±4.96)% and (79.85 ±5.44)%,respectively and which were better than those of the control group[(12.11 ±4.87)%,(30.24 ±1.53)%,(58.51 ±6.52)%](t=4.693,3.853,2.559,all P <0.05). According to the grading standards of the healing skin after HE staining ,the experimental group was better than the control group .According to the results of Western blot , the KGF protein expression of the experimental group was higher than the control group .Conclusion The allogeneic bone marrow mesenchymal stem cells may be an effective and safe method for the wound in rats .
