Effect of CD14 on lipid-associated membrane proteins of Mycoplasma genitalium activate NF-kappaB
10.3969/cjz.j.issn.1002-2694.2014.08.005
- VernacularTitle:CD14对生殖支原体LAMPs激活NF-κB的影响
- Author:
Jun HE
;
Yanhua ZENG
;
Xiaoxing YOU
;
Shaojian WU
;
Wei TIAN
;
Jun LIU
;
Yimou WU
- Publication Type:Journal Article
- Keywords:
CD14;
Mycoplasma genitalium;
lipid-associated membrane protein;
nuclear transcription factor κB;
hu-man serum
- From:
Chinese Journal of Zoonoses
2014;(8):797-799,859
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of CD14 on nuclear transcription factorκB (NF-κB) was activated by lipid-associated membrane proteins (LAMPs) of Mycoplasma genitalium (Mg) ,THP-1 cells were pretreated with serum human or CD14 neu-tralizing antibody ,and then were stimulated by LAMPs .The activation of NF-κBp65 was detected by ELISA .After LAMPs was pretreated with sCD14 stimulated Hela cells with the co-transfection ,the activity of NF-κB luciferase were detected by the dual-luciferase reporter gene to analyze the role of CD14-mediated NF-κB activation by LAMPs .The activation of NF-κBp65 was significantly up-regulated in LAMPs activated THP-1 cells by human serum .It’s suggested that CD14 neutralizing anti-body could inhibit the activation of NF-κBp65 in LAMPs stimulated THP-1 .The activation of NF-κB was significantly up-regu-lated in LAMPs activated Hela cells by mCD14 or sCD14 .CD14 could augment the activation of NF-κB by Mg LAMPs .
