High Performance Liquid Chromatograph Fingerprint of Liqi Xiaoying Tablets
10.3870/yydb.2014.08.024
- VernacularTitle:理气消瘿片高效液相色谱指纹图谱研究
- Author:
Junjie HU
;
Yi XIAO
;
Guihong WANG
;
Guohua ZHENG
- Publication Type:Journal Article
- Keywords:
Liqi xiaoying tablets;
Chromatography,fingerprint;
Chromatograph,high performance liquid
- From:
Herald of Medicine
2014;(8):1067-1069,1070
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish high performance liquid chromatograph ( HPLC ) fingerprint of liqi xiaoying tablets,and to provide reference for quality control of the herbal medication. Methods The chromatography conditions consisted of Aichrom Bond-AQ C18(250 mmí4. 6 mm,5 μm) column with mobile phase of acetonitrile-0. 1% phosphoric acid ( gradient elution) , column temperature of 30 ℃, flow rate of 1 mL · min-1 , injection volume of 20 μL, and UV detection wavelength of 226 nm. Results HPLC fingerprint was established with 13 common peaks,4 of which were identified. The similarity of the HPLC fingerprints of liqi xiaoying tablets from 10 batches was greater than 0. 95. Conclusion The method is accurate, reliable, and can reflect complete information for the quality of liqi xiaoying tablets.
