Construction of Salmonella Pullorum C79-13ΔcrpΔasd mutant balanced-lethal host-vector system and its biological features
10.3969/j.issn.1000-484X.2014.08.017
- VernacularTitle:鸡白痢沙门菌C79-13ΔcrpΔasd平衡致死系统的构建及其生物学特性的研究
- Author:
Songbiao CHEN
;
Jing LI
;
Ke SHANG
;
Chuan YU
;
Chunjie ZHANG
;
Xiangchao CHENG
;
Yinju LI
;
Zhanqin ZHAO
- Publication Type:Journal Article
- Keywords:
Salmonella Pullorum C79-13;
asd+balanced-lethal host-vector system;
Recombinant suicide vector;
Live vaccine vector
- From:
Chinese Journal of Immunology
2014;(8):1083-1087,1092
- CountryChina
- Language:Chinese
-
Abstract:
In order to develop a safer vaccine strain exploit Salmonella Pullorum vaccine strain as a live vaccine vector.Methods:AΔcrpΔasd mutant of S.pullorum C79-13 strain was constructed and it was developed E.coli donor strain mutant was generated through the two-step method introduced by χ7213 ( pREΔasd) was conjugated with the recipient of C 79-13Δcrp.The C79-13ΔcrpΔasd the transduction of recombinant suicide plasmid .Results:PCR and sequencing results showed that ΔsipBSL1344 was suc-cessfully constructed.The further study indicated that foreign DAP must be supplied for the ΔcrpΔasd mutant to grow,in addition,the asd gene was transmitted stably .Compared with C79-13 strain,the O antigens was identical to C79-13 strain,but the growth velocity was reduced significantly ,significantly reduced virulence .Conclusion: The ΔcrpΔasd mutant can accept asd+plasmid to construct host-vector balance lethal system , and this system can be used to express foreign gene efficiently and to develop potential oral multivalent vaccines.
