In Vitro Inhibition of Cytochrome P450 in Rats Liver Microsomes by Shuanghuanglian Injection Powder and Its Active Components
10.3870/yydb.2014.10.004
- VernacularTitle:双黄连粉针及其活性成分对大鼠细胞色素 P450酶的体外抑制作用
- Author:
Jing DAI
;
Licong WANG
;
Dan WU
;
Suiping TU
;
Liying QIU
- Publication Type:Journal Article
- Keywords:
Shuanghuanglian injection powder;
Baicalin;
Cytochrome P450;
Inhibition
- From:
Herald of Medicine
2014;(10):1269-1272,1273
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the inhibition effects of shuanghuanglian injection powder and its active components on the activities of CYP1A,CYP 2D and CYP 3A in rats liver microsomes. Methods Three probe substrates including phenacetin for CYP1A,dextromethorphan for CYP2D and midazolam for CYP3A were incubated with shuanghuanglian injection powder and the active components (baicalin,phillyrin,forsythiaside A,lutin,chlorogenic acid,coffeic acid and lutiolin) in rat liver microsomes. Contents of three probe substrates were simultaneously determined by HPLC to evaluate the metabolic rates. Results Shuanghuanglian injection powder and baicalin inhibited the activities of CYP2D and CYP 3A, but didn 't affect CYP1A. The other active components showed no effect on CYP1A,CYP2D and CYP3A. Conclusion Drug-drug interactions may occur when combining shuanghuanglian powder injection with CYP2D and CYP 3A substrates and baicalin may be the effector substance responsible for the interactions.
