Effects of taurine-magnesium coordination compound on abnormal calcium current induced by hypoxia-reoxygenation in rat ventricular cardiomyocytes
10.3969/j.issn.1001-1978.2014.10.009
- VernacularTitle:牛磺酸镁对缺氧/复氧致大鼠心肌细胞钙离子通道异常的影响
- Author:
Minghui ZHANG
;
Yongqiang YIN
;
Yi KANG
;
Jianshi LOU
- Publication Type:Journal Article
- Keywords:
taurine-magnesium coordination com-pound;
arrhythmia;
ventricular cardimyocytes;
calcium ion channel;
whole-cell patch clamp technique;
amiod-arone
- From:
Chinese Pharmacological Bulletin
2014;(10):1367-1371,1372
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of TMCC on abnormal L-type calcium current (ICa,L) in rat ventric-ular cardiomyocytes during hypoxia-reoxygenation to find out the mechanism of antiarrhythmic effect. Methods Whole-cell patch clamp was used to record ICa,L in the ventricular cardiomyocytes during hypoxia-reoxygenation in rat under amiodarone and different concentrations of TMCC. Results In hypoxia-reoxy-genation model, peak ICa,L increased from ( 3. 35 ± 0. 50 ) pA/pF to ( 5. 69 ± 0. 25 ) pA/pF ( n =6 , P <0. 01 ) , which was restored to ( 5. 28 ± 0. 18 ) pA/pF (n=6, P>0. 05),(4. 41 ± 0. 22) pA/pF, (3. 82 ± 0. 21)pA/pF(n=6, P<0. 01) by TMCC(100, 200, 400 μmol·L-1 ) and amidodarone 24. 24 μmol·L-1 restored peak ICa,L to(3. 66 ± 0. 27)pA/pF (n=6,P<0. 01 ) . Compared to control group, hypoxia-reoxy-genation turned ICa,L steady-state activation curves to left and inactivation curves to right, which quickened activation and slowed inactivation, TMCC ( 200, 400μmol · L-1 ) and amiodarone could restore the left shift activation curves and right shift inactivation curves. Conclusion TMCC can concentration-de-pendently restore the increase of calcium current due to hypoxia-reoxygenation by promoting inactivation process and inhibiting activation process, and the effect is equal to that of amiodarone. TMCC blocks ICa,L of the ventricular cardiomyocytes, which may be one of its antiarrhythmic mechanisms.
