Experimental study of PLCεon migration and invasion of human osteosarcoma cancer cells
10.3969/j.issn.1001-1978.2014.10.013
- VernacularTitle:PLCε对人骨肉瘤细胞迁移侵袭影响的实验研究
- Author:
Jinxia WU
;
Wenjia CAO
;
Miaomiao SANG
;
Junnian ZHENG
;
Dongsheng PEI
- Publication Type:Journal Article
- Keywords:
PLCε;
siRNA;
proliferation;
migration;
invasion;
osteosarcoma
- From:
Chinese Pharmacological Bulletin
2014;(10):1388-1391,1392
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of PLCε on the invasion and migration of human osteosarcoma cancer cells U2OS. Methods RNA interference ( RNAi) was used to inhibit PLCεexpression, and the proliferation of cancer cells was measured by CCK-8 assay. The migration of the cells was measured by scratch wound healing assay and migration chamber as-say. Gelatin zymography was performed to measure the MMP2 activities in U2OS cells. Results PLCε ex-pression was suppressed by siRNA. CCK-8 assay showed that PLCε had no effect on the proliferation of cancer cells. PLCε knockdown inhibited cell invasion and activities of MMP2 . Conclusion PLCε knock-down can inhibit the migration and invasion of human osteosarcoma cancer cells U2OS.
