Tuftsin-derivated TP affects MIP-1αexpression of tumor associated macrophages
10.3969/j.issn.1001-1978.2014.10.007
- VernacularTitle:Tuftsin衍生物TP影响肿瘤相关巨噬细胞 MIP-1α分子表达
- Author:
Yinghong AN
;
Na JIA
;
Linna LI
;
Su HAN
;
Dexuan YANG
;
Shoujun YUAN
- Publication Type:Journal Article
- Keywords:
immunopotentiator;
Tuftsin;
T peptide ( TP );
macrophage inflammatory protein ( MIP-1α);
residual tumor model;
tumor associated macrophages ( TAMs);
double tumor model
- From:
Chinese Pharmacological Bulletin
2014;(10):1357-1360,1361
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of TP on the expression of macrophages inflammatory protein ( MIP-1α) . Methods Total RNA of mouse Ana-1 cells and tumor associated macrophages were extracted, and MIP-1α mRNA was detected by RT-PCR. Mouse S180-xenografts were established by injecting S180 cells subcutaneously into the double abdominal flanks of the mice. The postoperative residual tumor models were generated in the right abdominal tumors when tumors grew into 250 mm3 . Animals were treated with TP or CTX, and tumor tissues were separated and MIP-1α was detected by immunohistochemistry. Results There was no significant difference of the expression of MIP-1α between Ana-1 cells and TAMs. TP couldn’ t affect MIP-1αexpression in Ana-1 cells while it signifi-cantly decrease MIP-1α expression in TAMs in a dose-dependent manner. TP significantly decreased MIP-1αexpression of tumor tissue compared with control group. Conclusions MIP-1α will be a new target of TP anti-cancer. Simple cell line tests in vitro couldn’ t reveal the real state in vivo.
