Quality Control for Tongsai Yinao Oral Liquids
- VernacularTitle:通塞益脑口服液的质量控制研究
- Author:
Haixiang REN
;
Jia JIA
;
Li FANG
;
Liye QIAO
;
Yin LU
;
Hua SU
- Publication Type:Journal Article
- Keywords:
Tongsai Yinao oral liquids;
TLC;
Total ferulic acid;
HPLC;
Determination
- From:
China Pharmacist
2014;(9):1489-1492,1493
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To improve the quality standard of Tongsai Yinao oral liquids for the quality control. Methods: TLC was used for the qualitative identification of Rhizoma corydails, Rhizoma chuanxiong and Rhizoma acori tatarinowii. HPLC was used to sim-ultaneously determine the content of total ferulic acid in the oral liquids. The determination was performed on a Lichrospher-C18 (250 mm×4.6 mm,5 μm)column with the mobile phase of methanol-0.1% H3PO4(30∶70). The flow rate was 1.0 ml·min-1,the col-umn temperature was at 30℃, the detection wavelength was 321nm and the injection volume was 20μl. Results:The spots on the TLC plates were clear without the interference of negative control. The linear range of ferulic acid was obtained between 2. 24 and 35. 84 μg ·ml-1(r=0. 999 9), and the average recovery was 102. 54%(RSD=0. 85%, n=6). Conclusion:The improved method is accu-rate and feasible. It can be used very conveniently in the quality control.
