Effect of increased serum TNF-αon lipid accumulation in kidney of diabetes
10.3969/j.issn.1001-1978.2014.08.025
- VernacularTitle:血清TNF-α升高对糖尿病肾脏脂质积聚的影响研究
- Author:
Song ZHANG
;
Lin ZHU
;
Jun HAO
;
Shushen ZHENG
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor-α;
diabetes;
renal tubular cells;
lipid accumulation;
adipose differentia-tion related protein;
sterol regulatory element binding protein-1
- From:
Chinese Pharmacological Bulletin
2014;(8):1161-1164,1165
- CountryChina
- Language:Chinese
-
Abstract:
Aim Todeterminetherelationshipbe-tween serum TNF-α and renal abnormal lipid metabo-lismindiabeticmice.Methods CD1micewerein-jected intraperitoneally with STZ (150 mg·kg-1 ) and the type 1 diabetic mice were determined with high fasting blood glucose ( >16. 7 mmol · L-1 ) 72 hours after injection. After fed for one month, normal control mice and diabetic mice were sacrificed. The serum TNF-α level was detected by the method of ELISA. Immunohistochemistry and Western blot were used to explore the expression of SREBP-1 and ADRP. Re-sults TheresultsofELISAshowedthatserumTNF-αwas increased by 7 . 73 times in diabetic mice compared with normal mice. SREBP-1 and ADRP expressions were located in renal tubular cells. Again, it was con-firmed by Western blot that SREBP-1 precursor seg-ment, SREBP-1 mature segment and ADRP were re-spectively enhanced by 2. 31 times, 1. 74 times and 1. 72 times in diabetic mice in comparison with normal control mice . In addition , the data analysis revealed a
positive correlation ( correlation coefficient 0. 914 ) be-tween serum TNF-αand renal ADRP expression. Con-clusion TheincreasedserumTNF-αmaybeoneof factors involved in renal lipid accumulation of diabe-tes.
