pIRES2-EGFP-NT3 transfected mouse cochlea fibroblast by lipofectamineTM 2000
10.3760/cma.j.issn.1008-1372.2014.07.009
- VernacularTitle:Lipofectamine TM 2000介导pIRES2-EGFP-NT3转染小鼠耳蜗成纤维细胞的实验研究
- Author:
Jie CHEN
;
Xia GAO
;
Xiaofeng MA
- Publication Type:Journal Article
- Keywords:
Cations;
Liposomes;
Transfection;
Genetic vectors;
Plasmids/genetics;
Cochlea;
Fibroblasts;
Mice
- From:
Journal of Chinese Physician
2014;(7):891-893,896
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the transfection of pIRES 2-EGFP-NT3 in mouse cochlea fibroblast by cationic liposome . Methods After pIRES2-EGFP-NT3 had been abstracted successfully , it was transfected into mouse cochlea fibroblast by lipofectami-neTM2000.Twenty four hours later, the efficiency of the transfection was analyzed by confocal microscope .Results The pIRES2-EG-FP-NT3 was effectively transfected into mouse cochlea fibroblast by cationic liposome .The transfected fibroblasts displaying green fluo-rescence were observed under fluorescence microscope .Conclusions The effective transfection of pIRES 2-EGFP-NT3 into mouse cochlea fibroblast by lipofectamine TM 2000 laid the basis for the following experiments , such as NT3 gene transfection in deaf or normal cochlea and so on .
