Preparation of cleavable PEG and RGD co-modified liposomes in vitro and evaluate its properties
- VernacularTitle:可断裂PEG与RGD共修饰脂质体的制备及其初步评价
- Author:
Feng YUE
;
Yijun JIA
- Publication Type:Journal Article
- Keywords:
cleavable PEG;
liposomes;
tumor targeting
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;37(4):56-58,62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the cleavable PEG and RGD co-modified liposome for tumor targeting.Methods Liposomes were prepared by film-ultrasonic method.The particle size,Zeta potential and stability in FBS were evaluated.Cellular uptake by HepG2 cell was explored.MTT assay was used to evaluate the cytotoxicity of blank liposomes. Results The particle diameter of C/RGD-LP was (104.8 ±5.5 )nm with the Zeta potential of (-4.45 ±1.75 )mV.The cellular uptake of C/RGD-LP increased 2.8 times after Cys was added.The C/RGD-LP showed little cytotoxicity to HepG2 cell.Conclusion Cleavable PEG and RGD co-modified liposomes were easy to prepare and has a special application value for targeting tumor.
