Study on the mechanism of peroxidant damage in liver induced by Traditional Chinese Medicine Euphorbia kansui
- VernacularTitle:中药甘遂致小鼠肝脏氧化损伤的机制研究
- Author:
Xiujuan ZHANG
;
Tong LU
;
Bo YANG
;
Qian DIAO
- Publication Type:Journal Article
- Keywords:
Chinese medicine Euphorbia kansui;
liver function;
enzyme activity;
oxidative damage
- From:
Chinese Journal of Biochemical Pharmaceutics
2014;37(4):63-65,69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanisms of Chinese medicine Euphorbia kansui induced liver damage. Methods 40 mices were selected and radomly divided into four groups according to the dose of Chinese medicine Euphorbia kansui used,there were 4 g/kg group(1/8 LD50 ),2 g/kg group(1/16 LD50),1 g/kg group(1/32 LD)50,and control group,which were given saline. After continuous administration for 21 d by ig,the body weight and liver coefficient of mice in all groups were determined,liver histological changes were detected by HE staining. The serum enzymatic activity of alanine aminotransferase (ALT)and aspartate aminotransferase (AST),the levels of superoxide dismutase (SOD)and glutathione peroxidase (GSH-Px),the content of malondialdehyde (MDA),superoxide anion radical (O2-·)and hydroxyl radical (OH·)were all measured by ultraviolet spectrophotometry. The changes of reactive oxygen species (ROS)content were detected by flow cytometry. Results With the increase dose of Chinese medicine Euphorbia kansui,the body weight of rats were decreased,and liver coefficient were increased(P<0.05). The serum ALT,AST activity were significantly increased,SOD and GSH-Px activity were significantly decreased(P<0.05),MDA content were increased significantly,radical O2-·, OH·and intracellular ROS levels were all increased (P<0.05 ). Conclusion Chinese medicine Euphorbia kansui induced liver injury though lipid peroxidation.
