Study on HLA-A,B,DRB1 high-resolution alleles polymorphism in Chongqing Han population
10.3969/j.issn.1671-8348.2014.26.017
- VernacularTitle:重庆汉族人群HLA-A、B、DRB1高分辨等位基因多态性研究
- Author:
Fang WANG
;
Qun LIAO
;
Xia HUANG
;
Tao ZHANG
;
Sumin ZHU
;
Hongmei LIAO
;
Xiaohong LI
;
Lei CHENG
;
Qianqian TAN
;
Hongli HUANG
;
Zhengli SONG
- Publication Type:Journal Article
- Keywords:
HLA antigens;
high-resolution typing;
gene frequency;
polymorphism;
Chongqing
- From:
Chongqing Medicine
2014;(26):3455-3457,3460
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the HLA-A ,B and DRB1 alleles high-resolution polymorphism in Chongqing Han population . Methods The PCR-SSOP and PCR-SBT methods were applied for the HLA high-resolution genotyping of 2 067 unrelated healthy donors in the registry of Chongqing branch of Chinese National Marrow Donor Program (CMDP) .The allele frequencies of HLA-A , B and DRB1 were estimated by the direct counting method and the Hardy-Weinberg equilibrium inspection was performed by using the Arlequin software 3 .1 .Results 168 high-resolution alleles were detected out ,in which 42 alleles of A*11 :01 ,A*24:02 ,A*02:07 ,A*02 :01 and A*33:03 at the HLA-A locus were observed with the frequencies greater than 0 .05 ;81 alleles were detected at HLA-B locus ,including B*46 :01 ,B*40:01 ,B*58 :01 ,B*13 :01 and B*15 :02 with the frequencies greater than 0 .05 ;45 al-leles of DRB1*09:01 ,DRB1*15 :01 ,DRB1*12 :02 ,DRB1*08 :03 and DRB1*11 :01 at the HLA-DR locus were observed with the frequencies greater than 0 .05 .Conclusion The data of the HLA-A ,B and DRB1allelic frequencies at high-resolution level in Chongqing Han population are obtained ,which provides the reliable reference data for the studies of anthropology ,forensic medi-cine ,transplantation matching and disease association .
