Effect of different sources of tolerogenic dendritic cells on root resorption and healing after tooth transplantation in rats
10.3969/j.issn.1671-8348.2014.26.019
- VernacularTitle:不同来源致耐受DC对大鼠牙移植后牙根吸收及愈合影响的研究
- Author:
Linhong ZOU
;
Linlin ZHANG
;
Yurong WANG
;
Chuncheng SHAN
;
Zhiqiang MING
;
Yunjia CHEN
;
Wenying LI
- Publication Type:Journal Article
- Keywords:
tooth replantation;
dendritic cells;
periodontal ligament;
root resorption;
healing
- From:
Chongqing Medicine
2014;(26):3461-3463,3467
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of different sources of dendritic cells (DCs) on root resorption and healing after allo-geneic tooth transplantation in rats .Methods The BN rats and Lewis rats were respectively used as the donor and recipient teeth for establishing the animal experimental transplantation models and randomly divided into 4 groups ,20 cases in each group :the syn-geneic transplantation group(A) and the allogeneic transplantation groups(B ,C ,D) .The group A and B were infused with PBS buffer solution 0 .5 mL on 7 d before operation .The group C and D were infused with 1 × 106 donor or recipient tolerogenic DCs on 7 d before operation .5 rats randomly selected from each group were sacrificed for performing the pathological examinations of transplanted teeth at the end of 1 ,2 ,4 and 8 weeks .Results The inflammatory cellular infiltration was most severe in the group B , slightest in the group A and moderate in the group C and D .The root resorption was minimal in the group A ,maximal in the group B and moderate in the group C and D (P<0 .05);the root resorption at 2 ,4 weeks had no statistical difference between the group C and D(P>0 .05) ,but which at 8 weeks in the group D was lower than that in the group C .The periodontal membrane healing points at 8 weeks in the group C and D was less than that in the group A ,but more than that in the group B (P<0 .05);the inflam-matory absorption was highest in the group B(P<0 .05);the alternative absorption was lowest in the group A (P<0 .05) .Conclu-sion The two different sources of tolerogenic DCs all could reduce the rejection reaction of allogeneic tooth transplantation ,reduce the root resorption and promote the healing of periodontal membrane .Recipient tolerogenic DCs could reduce the root resorption in late stage even more .
