Research for protective effect of Fluoxetine in experimental autoimmune enceph-alomyelitis
10.3969/j.issn.1000-484X.2014.08.007
- VernacularTitle:氟西汀在实验性自身免疫性脑脊髓炎中的保护作用研究
- Author:
Yu ZHANG
;
Guiquan WANG
;
Meini ZHANG
- Publication Type:Journal Article
- Keywords:
Experimental autoimmune encephalomyelitis;
LCN2;
CXCL10;
Multiple sclerosis;
Fluoxetine
- From:
Chinese Journal of Immunology
2014;(8):1036-1039,1045
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the correlation between LCN 2 or CXCL10 and experimental autoimmune encephalomyelitis(EAE),and the effect of Fluoxetine in EAE mice.Methods: Mice were randomly divided into four groups: control group, EAE group, intervention group and fluoxetine group , each group included twenty mice.EAE model were constructed with MOG35-55 in the intervention group and fluoxetine group ,following ultraviolet (UV) (280-320 nm) irradiation.Mice in fluoxetine group were given fluoxetine (10 mg/kg) by daily gavage since immunized ,and the saline was used in the control group ,intervention group and EAE group in the same way and same time.The drug/saline was continuously administered from the immunization to the day mice were sacrificed.The mean attacked time ,the nerve function grades and the incidence were observed and compared.We observed brain pathological changes by HE staining and immunohistochemistry;CXCL10 were tested by ELISA method ,and compared among the four groups.Results:The mean attacked time ,the nerve function grades and the incidence in the fluoxetine group were lower than those in the intervention group and EAE group ,there were significant differences between two groups (P<0.05),in the intervention group were lower than those in the EAE group ,there were significant differences between two groups ( P<0.05 ).Fluoxetine treated EAE mice showed decreased degree of inflammatory infiltration;the mean rank of positive cells lower in the fluoxetine group than those in the EAE group,the differences were significant between two groups ( P<0.05 ).The expression of CXCL10 in the peripheral blood plasma of fluoxetine group were lower than those in the EAE group ,there were significant differences between two groups ( P<0.05 ).Levels of LCN2 expression were correlated with those of CXCL 10 in the EAE group.Conclusion: LCN2, CXCL10 are correlated with the pathogenesis of EAE.Fluoxetine could alleviate clinical symptoms of EAE and alleviate the morbidity of EAE.
