Interaction of PLGA nanoparticles with HL60 cells by fluorescence tracking
10.3760/cma.j.issn.1673-4181.2014.03.005
- VernacularTitle:荧光示踪PLGA纳米粒与HL60细胞间相互作用
- Author:
Li LU
;
Feng LYU
;
Li WU
;
Tianjun LIU
- Publication Type:Journal Article
- Keywords:
PLGA nanoparticles;
Fluorescence tracking;
HL60 cell;
Transmembrane process
- From:
International Journal of Biomedical Engineering
2014;37(3):148-150,162,后插6
- CountryChina
- Language:Chinese
-
Abstract:
Objective Nanoparticles are widely investigated and applied in clinical diagnosis and treatment as drug carrier,and their transmembrane process is related to their biological effects.The aim of this study was to investigate the interaction of fluorescein-labeled PLGA nanoparticles and HL60 cells via fluorescence tracking.Methods The transmembrane process of nanoparticles was quantitatively analyzed by laser scanning confocal microscopy.Results The analyzing results showed that the interaction of fluorescein-labeled PLGA nanoparticles and HL60 cells was strongly temperature-dependent.The receptor-mediated endocytosis mechanism played an important role in the transmembrane process for cellular uptake of nanopaticles.Conclusions This study provides a theoretical basis for design and application of nano-medicines.
