Dose effect of laser irradiation in photodynamic therapy for laryngocarcinoma in BALB/c mouse
10.3760/cma.j.issn.1673-4181.2014.03.003
- VernacularTitle:PDT治疗BALB/c荷瘤小鼠喉癌中的激光剂量效应研究
- Author:
Huijuan YIN
;
Minghui WANG
;
Hongli CHEN
;
Zengrui XU
;
Yingxin LI
- Publication Type:Journal Article
- Keywords:
Photodynamic therapy;
Laryngocarcinoma;
Hematoporphyrin monomethyl ether;
Laser dose;
Hep-2
- From:
International Journal of Biomedical Engineering
2014;37(3):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The dose and type of light and photosensitizer could seriously affect the curative effect of photodynamic therapy (PDT).The purpose of this study was to observe whether or not PDT with hematoporphyrin monomethyl ether (HMME) can cure laryngocarcinoma in the solid tumor model,and to define the proper laser amount for killing the cancer cells.Methods Forty eight BALB/c mouse models with subcutaneous Hep-2 laryngeal carcinomas were prepared.Mice were divided into six groups depending on the amount of laser received from 30 J/cm2 to 480 J/cm2 including a control group,tumor size in each group was between 8 mm and 10 mm.Tail vein injection were given with HMME prior to applying the laser light,and then illumination was carried out on the tumor at 3 h after HMME administration.Tumor volume,animal weight and histopathologic changes were observed after PDT.Results All mice apparently showed positive results via PDT,and the cancer had been cured in 120 J/cm2 and 480 J/cm2 groups.The laryngeal cancer lesions began to form scab 1 d after PDT and the scab became hard and black after 5 d.The tumor regression began simultaneously and completed around 30 d after PDT.Conclusions PDT may treat laryngeal cancers which sized less than 10 mm in mouse models.The optimum energy to destruct the laryngeal cancer cells may be 120 J/cm2.
