Preparation of brain active-targeting endomorphin loaded nanoparticles and study on its effects of passing across blood brain barrier
10.3760/cma.j.issn.1673-4181.2014.03.002
- VernacularTitle:OX26偶联载内吗啡肽超支化聚甘油-聚乳酸-聚乙醇酸纳米粒透血脑屏障机制实验研究
- Author:
Xu JIN
;
Wei XIONG
;
Min ZENG
;
Feng LYU
;
Hanmei BAO
;
Tianjun LIU
- Publication Type:Journal Article
- Keywords:
Nanoparticle;
Active-targeting;
Pain therapy;
Blood brain barrier;
Endocytosis
- From:
International Journal of Biomedical Engineering
2014;37(3):135-138,后插3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a novel brain active-targeting endomorphin (EM) loaded hyperbranched polyglycerols-poly (lactic-co-glycolic acid) (HBPG-PLGA) nanoparticles (NPs) and study its mechanism of passing across blood brain barrier (BBB) in brain microvascular endothelial cells (BMEC).Methods The OX26 (transferring receptor monoclonal antibody) conjugated EM loaded HBPG-PLGA NPs was constructed according to water-in-oil-in-water emulation solvent evaporation technique as a novel biodegradable brain active-targeting drug delivery system.The properties of the NPs were evaluated by transmission electron microscope (TEM) in vitro.Through flow cytometry and laser scanning confocal microscope,the mechanism of passing across BBB was evaluated.Results The preparation methodology of NPs was optimized and established.The mean diameter was (170±20) nm and Zeta potential was about-27 mV.Core-shell construction was showed on TEM.Cellular uptake study showed that the uptake of NPs was via a caveolae-mediated endocytic pathway,then endomorphin and carrier were divided into two parts in BMEC.Conclusions The OX26 conjugated EM loaded NPs were stable,and demonstrate remarkable effects on crossing BBB.Cellular uptake by BMEC is a very important mechanism of the NPs' brain activating-targeting effect.
