A novel way for neurodegenerative diseases within cell and animal model by autophagy induced with rapamycin treatment
10.3760/cma.j.issn.1673-4181.2014.03.001
- VernacularTitle:雷帕霉素通过诱导细胞自噬在细胞和动物模型中治疗神经退行性疾病的新思路
- Author:
Xiaoming LI
;
Xudong ZHANG
;
Lin MEI
;
Laiqiang HUANG
- Publication Type:Journal Article
- Keywords:
Rapamycin;
Neurodegenerative diseases;
Cell autophagy;
Mitochondria damage;
Apoptosis
- From:
International Journal of Biomedical Engineering
2014;37(3):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using rapamycin protecting neurons' mitochondrial within injury model of neurodegenerative disease thus to reduce neuronal damage.Methods Parkinson's disease (PD) injury model of cells and animals were established,then rapamycin was added,and induced autophagy,the polarity changes of mitochondrial membrane potential,intracellular reactive oxygen species (ROS) scavenging and the contents of NF-κB inside the nucleus after dyeing were observed by confocal laser scanning microscopy.Results Rapamycin can induce autophagy and maintain morphology of mitochondria in cells damaged by MPP+.Mitochondrial membrane potential,level of ROS and contents of NF-κB inside the nuclear can be detected obviously reduced in these cells with rapamycin' s function.Furthermore,rapamycin also protects neurons well within PD models of zebrafish and mouse which are damaged by MPTP.Conclusions Rapamycin induces autophagy and protects mitochondria from damage within neurodegenerative disease,and strengthening this kind of protection of mitochondria is a novel way to deal with neurodegenerative diseases.
