B7H3 upregulates neuron-specific enolase and S100b mRNA expression in mice with S .Pneumoniae meningitis
10.3969/j.issn.1671-8348.2014.19.014
- VernacularTitle:B7同源体3上调肺炎链球菌脑膜炎小鼠NSE和S100bmRNA的表达
- Author:
Yanping WANG
;
Xuqin CHEN
;
Zhedong WANG
;
Bingbing ZHANG
;
Fengqing FU
;
Yan LI
- Publication Type:Journal Article
- Keywords:
meningitis,pneumococcal;
brain injuries;
B7H3;
neuron-specific enolase;
S100b
- From:
Chongqing Medicine
2014;(19):2437-2439,2442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of B7 Homology 3(B7H3)on brain damage of S .Pneumococcal(SP)meningitis . Methods SP meningitis was established by intracerebral ventricular injection of SP suspension on wild-type BALB/C mice .48 mice were divided into 4 groups and received following injections :NS(CON group) ,recombinant murine B7H3alone(B7H3 group) ,SP group ,SP+B7H3 group .At 18 ,48 ,72 h post infection ,mice were conducted neurobehavior score ,then they were anesthetized and killed by cervical vertebra dislocation ,brains were collected .The mRNA expressions of NSE and S100b were detected by real-time PCR .Results Compared with CON group ,the scores of recombinant murine B7H3 group had no significant change at 18 ,48 ,72 h after infection of SP(P>0 .05);at different time points the scores of SP group were decreased significantly than the CON group (P<0 .05);scores of SP+B7H3 group decreased furtherly than SP group(P<0 .05) .The relative expressions of NSE ,S100b mR-NA in brain tissue homogenate :for the NSE ,S100b mRNA relative expressions ,there was no significant difference between B7H3 and CON group at 18 ,48 ,72 h post SP injection(P>0 .05) .At 18h ,48h ,72h ,post infection mRNA expressions of NSE ,S100b in SP group increased compared with CON group(P<0 .05);the mRNA expressions of NSE ,S100b increased furtherly in SP+B7H3 group compared with SP group(P<0 .05) .Conclusion B7H3 upregulates the mRNA expressions of NSE and S100b ,and promotes the progress of SP meningitis in mice .
