The value of genotype detection for diagnosis of hepatitis B virus infection
10.3969/j.issn.1671-8348.2014.20.005
- VernacularTitle:基因型检测对诊断乙型肝炎病毒感染程度的价值
- Author:
Yi WU
;
Lin GAN
;
Cunyan LI
;
Yan JIANG
;
Yuan LI
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
genotypes;
gene;
reverse dot hybridization;
polymerase chain reaction
- From:
Chongqing Medicine
2014;(20):2557-2558,2562
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the value of genotype detection for diagnosis of hepatitis B virus (HBV ) infection . Methods 433 HBV pattients from January 2011 to August 2013 were detected by PCR-reverse dot blot hybridization ;the DNA were assaied by PCR ;the HBeAg were tested by ELISA .Results Of the 432 HBV patients ,the rato of genotypes B (68 .13% ) was significantly higher than that of genotypes BC (5 .77% ) and of genotypes C(26 .10% )(P<0 .05);there were no significiant differ-ence in the copy of the HBV DNA among the various genotype (P>0 .05);HBeAg negative rate of genotypes B (23 .82% ) ,geno-types BC(14 .78% ) ,genotypes C(1 .42% )had statistically significant(P<0 .05);Genes associated with disease severity :the ratio of genotype B for patients with mild-to-moderate hepatitis B was 87 .20% ,the rato of genotype C was 9 .34% and genotype BC was 3 .46% ,while the ratio of genotype C was 77 .08% ,genotype BC was 14 .58% ,genotype B was 8 .33% in severe hepatitis B .Con-clusion The genotype of HBV is related to disease severity and the negative rate of HBeAg ,it is not associated with HBV DNA of HBV .
