Comparison in purification methods of the recombinant human cardiac troponin I
10.3969/j.issn.1673-4130.2014.14.001
- VernacularTitle:重组人心肌肌钙蛋白I纯化方法的对比
- Author:
Qingqin HAO
;
Jianping ZHOU
;
Xiuli XU
;
Pei LIU
;
Xinyu WEN
;
Ling WANG
;
Yaping TIAN
- Publication Type:Journal Article
- Keywords:
cardiac troponin I;
dilution refolding;
column refolding;
purification
- From:
International Journal of Laboratory Medicine
2014;(14):1817-1818,1820
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the two kinds of purification method for purifying recombinant human cardiac troponin I(cT-nI)to obtain the stable cTnI and promote the study of cTnI diagnosis standardization.Methods The cTnI inclusion body was ob-tained by the ultrasonic broken engineering,after washing by 2% Tritonx-100,2M urea,dissolved in 8M urea,then purified by the column refolding on CM-FF and the dilution refolding respectively.The cTnI yields were compared between the two kinds of meth-od and the stability at 4 ℃,20 ℃,-80 ℃ and on the freeze-dried condition was compared.Then the purification method to effi-ciently obtain the stable cTnI was established.Results The protein about 2 mg and 1.4 mg could be obtained by CM-FF on the col-umn refolding and the dilution refolding from 0.1 g of wet inclusion body,respectively.The former method had the short cycle and high efficiency.The cTnI purified by the column refolding on CM-FF was more stable at 4 ℃,20 ℃,-80 ℃ and on the freeze-dried condition.Conclusion The column refolding on CM-FF is more stable and highly efficient in purification of cTnI than the dilution refolding.
