Research on influence of human cytomegalovirus infection on expression of vascular smooth muscle cell lipid metabolism-related gene
10.3969/j.issn.1673-4130.2014.14.003
- VernacularTitle:人类巨细胞病毒感染对血管平滑肌细胞脂代谢相关基因表达的影响
- Author:
Mingying XIE
;
Jingsu LI
- Publication Type:Journal Article
- Keywords:
human cytomegalovirus;
vascular smooth muscle cells;
cholesterol;
umbilical artery
- From:
International Journal of Laboratory Medicine
2014;(14):1821-1822,1824
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of intracellular cholesterol lipid metabolism and related gene expression after vascular smooth muscle cells infection by human cytomegalovirus(HCMV).Methods By separating the primary umbilical artery vascular smooth muscle cells with a multiplicity of infection of HCMV attack as the experimental group and the control group a-dopted the DMEM/F12 medium containing 3% bovine serum for simulating infection.The change of cholesterol content in the smooth muscle cells after virus attack was detected and the gene expression gene was detected by the gene expression profiling chip method.Results After vascular smooth muscle cells were infected by HCMV,the intracellular gene expression changed significant-ly,in which low-density lipoprotein receptor-related protein 10,11,12 and HMG-CoA synthase,HMG-CoA reductase and B scaven-ger receptor were upregulated.Apolipoprotein A1 and apolipoprotein M were downregulated.Conclusion HCMV infecting vascular smooth muscle cells may alter the lipid metabolism pathway related gene expression,enhance intracellular cholesterol synthesis,re-sult in the cholesterol metabolism imbalance,thus participate in the pathogenesis process of atherosclerosis.
