Study on transdifferentiation-acquiring tissue stem cell potency during renal tubular epithelial cells inflammatory damage
10.3969/j.issn.1673-4130.2014.14.005
- VernacularTitle:肾小管上皮细胞炎性损伤中的转分化-获得组织干细胞潜能的研究
- Author:
Lei PI
;
Tang JIANG
;
Bin HUANG
;
Juan OUYANG
;
Peisong CHEN
;
Yingpeng CUI
;
Yunfeng LIU
;
Caijiao GUO
- Publication Type:Journal Article
- Keywords:
renal tubular epithelial cell;
transdifferentiation;
Pax2;
CD133
- From:
International Journal of Laboratory Medicine
2014;(14):1825-1826,1829
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the potency of transdifferentiated renal tubular epithelial cells for acquiring the tissue stem cells during renal fibrosis.Methods The in vitro cellular model of renal tubular epithelial cells(NRK-52E)transdifferentiation under the inflammatory environment of the local renin-angiotensin (AngⅡ)system was established.The expression and change situation of the embryonic kidney developmental gene Pax2 and the tissue stem cell surface marker CD133 were observed.Results Local high concentration of AngⅡcould stimulate the NRK-52E cells to express Pax2 and CD133 molecule,its effect demonstrated the dose-and time-dependent relation.Conclusion The inflammatory damage leads to the transdifferentiated renal tubular epithelial cells po-tency to acquire the tissue stem cell.
