More reliable erythrocyte parameter MCH than MCV for screening thalassaemia trait
10.3969/j.issn.1673-4130.2014.14.041
- VernacularTitle:红细胞参数中 MCH 指标筛查地中海贫血比 MCV 指标更可靠
- Author:
Lianxiang LI
;
Qizhi XIAO
;
Yuqiu ZHOU
- Publication Type:Journal Article
- Keywords:
thalassemia;
screening;
mean corpuscular volume;
mean corpuscular hemoglobin
- From:
International Journal of Laboratory Medicine
2014;(14):1914-1915,1918
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the reliability of erythrocyte parameter mean corpuscular volume(MCV)and mean corpuscu-lar hemoglobin(MCH)for screening thalassaemia trait.Methods A fresh venous blood sample with the cut-off value of MCV was sent to 21 hospitals of Zhuhai city for conduct the full blood cell analysis within 1 d.Then the inter-and intra-coefficient of varia-tion (CV)as well as bias values of MCV and MCH were calculated and compared.In addition,10 EDTA-anticoagulant venous blood samples were divided into two parts,the effects of stored temperature and time on MCV and MCH were observed.Results The coefficient of variation(CV)of MCV (4.1%)was significantly greater than that of MCH (2.8%)among 21 laboratories,the qualification rate of MCH detection results was 100%,which was significantly higher than 66.7% of MCV (P <0.05).When the whole blood samples were stored under 2 kinds of the temperature condition(room temperature and refrigeration)for 72 h,MCH changed little (P >0.05).When these samples were stored under the refrigerated condition for 72 h,MCV had no statistically sig-nificant difference compared with the instant detection results of MCV (P >0.05),when stored at the room temperature for 48 h, MCV was significantly increased (P <0.05),MCV had statistical difference in storage for 48 h between the room temperature and the refrigeration.Conclusion Among laboratories and under different temperature conditions,the reproducibility of MCH is better than that of MCV and is more stable than MCV.MCH as the clinical first-line screening for thalassaemia is more reliable than MCV.
