Analysis of glycosylation hemoglobin by capillary electrophoresis
10.3969/j.issn.1673-4130.2014.14.043
- VernacularTitle:毛细管电泳法分析糖基化血红蛋白
- Author:
Jie MIAO
;
Weiling FU
;
Zhenwei QIU
;
Xuehui LI
;
Jingsheng WANG
- Publication Type:Journal Article
- Keywords:
capillary electrophoresis;
glycosylation hemoglobin;
detection
- From:
International Journal of Laboratory Medicine
2014;(14):1919-1920,1922
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the application value of the capillary electrophoresis in detecting the glycosylation hemoglobin (HbA1c)level.Methods By utilizing the uncoated fused silica capillary column(30 cm in length and 25 μm in diameter)and the specific adsorption peak,the HbA1c levels in the healthy people,patients with diabetes mellitus(DM)and high risk people were de-tected by the photodiode array(PDA)method at the wavelength of 415 nm.During the research process,the precision and accuracy were perfomred the accuracy analysis.Results HbA1c could be effectively separated from non-HbA1c,with a sharp peak.And the relative quantity of HbA1c could be determined.Conclusion The capillary electrophoresis is characterized by specificity,high precision,and high accuracy.Therefore,it should be widely applied in clinic.
