Application value and quality control of immunocytochemical staining in diagnosis of breast cancer
10.3969/j.issn.1673-4130.2014.14.045
- VernacularTitle:免疫细胞化学染色在乳腺癌诊断中的应用价值及质量控制
- Author:
Caibiao HE
;
Tao WU
;
Yaping ZHU
;
Hong YE
;
Zhiyong HU
;
Lin WANG
;
Yong CHEN
;
Bei WEI
- Publication Type:Journal Article
- Keywords:
breast cancer;
immunohistochemistry;
quality control
- From:
International Journal of Laboratory Medicine
2014;(14):1923-1924,1927
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application value and quality control of breast cancer cells PR,ER C-erbB-2,P53,Ki67 expressions in the patients with breast cancer.Methods The specimens were obtained by the fine needle aspiration cytological smear or intraoperative imprint cytology.The biological expressions of ER,PR,C-erbB-2,P53,Ki67 were detected by the immuno-cytochemical staining,the detection results with those detected by the immunohistochemical staining were performed the compara-tive analysis.Results The biological expressions of ER,PR,C-erbB-2,P53,Ki67 in 53 cases of breast cancer were 73.56%, 58.49%,56.60%,68.63%,81.13% respectively;ER,PR,C-erbB-2,P53,Ki67 had no statistical significant differences in positive cases and positive intensity between the immunocytochemical staining and the immunohistochemical staining (P >0.05).Conclusion The immunocytochemical staining can replace the immunohistochemical staining and has the important significance in the preop-erative diagnosis of breast cancer,therapeutical scheme selection and prognosis judgment.The whole process quality control is the key to do the breast cancer immunocytochemical staining well.
