Effects of ischemic postconditioning on nitric oxide and nitric oxide synthase in diabetic rat brain tissues
10.3760/cma.j.issn.1008-1372.2014.06.004
- VernacularTitle:缺血后处理对糖尿病大鼠脑组织 NO及 NOS 含量的影响
- Author:
Yingyun LU
;
Guangjun ZHAO
;
Yanna YANG
;
Ying LIU
;
Cuilan WANG
- Publication Type:Journal Article
- Keywords:
Reperfusion injury/prevention&control;
Diabetes mellitus;
Nitric oxide/metabolism;
Nitric oxide synthase/me-tabolism;
Rats,Wistar
- From:
Journal of Chinese Physician
2014;(6):731-733,738
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of ischemic postconditioning on the nitric oxide ( NO) and nitric oxide synthase ( NOS) in diabetic rat brain tissues .Methods Thirty Wistar rats were diabetic models induced by intraperitoneal injuction of stepto-zotocin (STZ), and randomly divided into three groups: Control group (normal, diabetic), cerebral ischemia group, and ischemic postconditioning ( I-POST) group.The rats of cerebral ischemia group and ischemic postconditioning group were made model of cere -bral ischemia by ligation carotid artery .Hematoxylin-eosin ( HE) was used to observe their pathological changes in control and diabetic groups.Enzyme-linked immunosorbent assay ( ELISA) method was used to detect the expression and changes of NO and NOS in the sera in each group .Western Blot method was used to investigate the expression and changes of NOS in the retinal tissues in each group .Results For I-POST group , brain tissue defects were decreased , neuronal cells were increased , serum inducible NOS ( iNOS) content was significantly lower than endothelial NOS (eNOS) and neuronal NOS (nNOS) ( P <0.05), brain tissue iNOS expression was significantly weaker than ischemia group ( P <0.05 ) and was not different from normal group ( P >0.05 ) .Conclusions Is-chemic postconditioning can protect the brain tissue of diabetic rats by inhibiting NOS activity especially iNOS .
