Determination of Related Substances in Vinpocetine Injections by HPLC
- VernacularTitle:HPLC测定长春西汀注射液中4种有关物质含量
- Author:
Yujia ZHANG
;
Li XIANG
;
Shaohua CHEN
;
Wensheng ZHENG
- Publication Type:Journal Article
- Keywords:
Vinpocetine injections;
Related substances;
HPLC
- From:
China Pharmacist
2014;(2):234-237,238
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for determining the contents of related substances in vinpocetine injections. Methods:The chromatographic separation was performed on a Kromasil C18 column (150 mm × 4. 6 mm,5 μm). The mobile phase consisted of 0. 2 mol·L-1 ammonium acetate -acetonitrile (40∶60), the flow rate was 1. 0 ml·min-1, the detection wavelength was 280 nm,and the injection volume was 20μl. Results:Vinpocetine and the related substances such as ethyl vincaminate, apovincamine, methoxyvinpocetine and dihydrovinpocetine were separated completely. The calibration curves of related substances showed good linear-ity. The average recoveries of related substances were all above 99. 8%. Conclusion:The method is accurate, sensitive and specific, and can be applied in determining the related substances in vinpocetine injections.
