Determination of Dexamethasone in Verdihong Paints by HPLC
- VernacularTitle:HPLC法测定维地红涂剂中地塞米松
- Author:
Yubo XIE
;
Haowan HUANG
;
Ling LI
;
Mingquan CHANG
- Publication Type:Journal Article
- Keywords:
Verdihong paints;
Dexamethasone;
Content determination;
HPLC
- From:
China Pharmacist
2014;(2):320-321,322
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of dexamethasone in Verdihong paints to ensure safety and ef-fectiveness of the clinical medicines. Methods:An HPLC method with a Dikma C18 column (150 mm × 4. 6 mm, 5 μm) was used, the mobile phase was a mixture of methanol-water-glacial acetic acid (80∶20∶0. 5), the detection wavelength was at 240nm, the injec-tion volume was 20 μl, the column temperature was 30℃ and the flow rate was 1. 0 ml·min-1 . Results:The standard curve of dexa-methasone was linear over the range of 3. 0-96. 0μg·ml-1(r=0. 999 7). The average recovery was 99. 45% with RSD of 1. 01%(n=9). Conclusion:The method is simple with good reproducibility, and can be used in the determination of dexamethasone in Verdihong paints.
