Determination of Loganin in Tiaojing Zhuyun Granules by HPLC
- VernacularTitle:HPLC法测定调经助孕颗粒中马钱苷的含量
- Author:
Liu YANG
;
Dapeng LIU
;
Yisheng ZHANG
;
Caixin ZHOU
- Publication Type:Journal Article
- Keywords:
Tiaojing Zhuyun granules;
Loganin;
HPLC
- From:
China Pharmacist
2014;(2):324-325,326
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a determination method for loganin in Tiaojing Zhuyun granules by HPLC. Methods: HPLC was used with a SinoChrom ODS-BP (250 mm × 4. 6 mm,5μm) as the analytical column. The mobile phase was composed of acetoni-trile-0. 1% phosphoric acid solution(14∶86). The detection wavelength was set at 240 nm and the column temperature was 30℃. Re-sults:The calibration curve of loganin was in a good linearity over the range of 50. 40-1 008. 00 ng(r=0. 999 9). The average recov-ery was 99. 33%(RSD=1. 41%, n=6). Conclusion:The method is simple,feasible and reproducible,and can be used in the quality control of Tiaojing Zhuyun granules.
