Determination of Astragaloside in Bushen Wenfei Mixtures by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定补肾温肺合剂中黄芪甲苷的含量
- Author:
Chuanping FENG
;
Hui YANG
;
Qingfeng DI
;
Bin CHEN
- Publication Type:Journal Article
- Keywords:
Bushen Wenfei mixtures;
Astragaloside;
HPLC-ELSD
- From:
China Pharmacist
2014;(2):322-323,324
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the content determination method for astragaloside in Bushen Wenfei mixtures by HPLC-ELSD. Methods:HPLCwithanAgilentZORBAXC18(150mm×4.6mm,5μm)columnwasused. Methanol-water(75∶25)wasusedasthe mobile phase and the flow rate was 0. 8 ml·min-1 . The drift tube temperature was 80℃ and the gas flow rate was 2. 5 ml·min-1 . Results: The linear range of astragaloside was 0.297-4.944 μg (r =0.999 7). The average recovery was 98.13% with RSD of 1. 27%(n=9). Conclusion:The method is with sufficient accuracy, stability and repeatability, and can be used in determination of Bushen Wenfei mixtures.
