Expression of B7-H4 in experimental autoimmune myocarditis
10.3969/j.issn.1000-484X.2014.06.006
- VernacularTitle:B7-H4在实验性自身免疫性心肌炎小鼠中的表达
- Author:
Zhongliang LIU
;
Ge LI
;
Yanyan SONG
;
Bin CUI
;
Haitao YUAN
- Publication Type:Journal Article
- Keywords:
B7-H4;
Costimulatory-molecules;
Experimental-autoimmune-myocarditis
- From:
Chinese Journal of Immunology
2014;(6):745-748,753
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the expression of B7-H4 in experimental autoimmune myocarditis (EAM).Methods:BALB/c mice were randomly divided into 2 groups:the control group and the experimental group.The mice of experimental group were injected with myosin to establish EAM models , while the mice of control group were injected with complete Freund 's adjuvant and normal saline.All the mice were killed separately at the 14th,21st,30th and 45th day for lymphocyte proliferation assay ,hematoxylin-eosin staining,immunohistochemical staining and real-time PCR.Results:The inflammation infiltration of heart was most serious at the 14th and 21st day,then it was gradually relieved with time;the results of lymphocyte proliferation assay and real-time PCR were similar to that of the inflammation infiltration of heart ,which were in high level at the 14th and 21st day,and they were both higher than that of the control group ( P<0.05 );B7-H4 protein were only detected in the experimental group ,and it was constantly expressed during the whole experiment on the endothelium of heart with myocarditis.Conclusion:B7-H4 participates in the progress of EAM ,and it may be a new way of studying the mechanism of myocarditis.
