Ultrastructural observation of dormant mouse embryos cultured in vitro after freezing-thawing
10.3969/j.issn.1005-4847.2014.03.011
- VernacularTitle:冻融后小鼠休眠胚胎超微结构的变化
- Author:
Meichao GU
;
Tiangang LU
;
Yunhai LIU
;
Hemin NI
;
Shaoyu ZHANG
;
Chundong ZHAI
;
Shuhan XING
;
Yong GUO
- Publication Type:Journal Article
- Keywords:
Mouse;
Dormant embryo;
Ultrastructure;
Freezing-thawing
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(3):53-56,61
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to investigate the differences of the cell ultrastucture of normal mouse hatched blastocysts and their dormant ones cultured in vitro after freezing-thawing, and to explore whether the dor-mant embryos have a better anti-freezing shock property than the normal hatched mouse embryos .Methods By transmis-sion electron microscopy , the ultrastructure of these two types of mouse embryos was observed and analyzed .Results By comparative analysis of their ultrastructure , the results showed that the dormant embryos before freezing are being austerity and with lower energy metabolism at a ‘ground state ’ .After freezing-thawing and culture , their cellular structure seemed to be similar to that of the normal embryos cultured in vitro before freezing.However, after freezing-thawing and culture, the number of mitochondria decreased , the nuclei were loose , and their heterochromatin also increased .Conclusions From the ultrastructural observation , compared with the normal mouse hatched embryos , the cellular state of dormant mouse em-bryos after freezing-thawing is more favorable for material storage and energy metabolism , thus, indicating that they have a better anti-freezing property than normal hatched embryos .
