Experimental study of correlation between T-cell paradigm and bone loss induced by estrogen deficiency
10.3969/j.issn.1000-484X.2014.07.002
- VernacularTitle:T细胞亚群偏移与雌激素缺乏骨丢失相关性实验研究
- Author:
Xiulian GAO
;
Meiyun XING
;
Li WANG
;
Yuqi GUO
;
Xianbin ZHOU
;
Qiaofeng ZHANG
;
Honghai ZHANG
;
Chengfang YAO
;
Guosheng JIANG
;
Xia LI
- Publication Type:Journal Article
- Keywords:
Estrogen deficiency;
T-cell paradigm;
Boss metabolism;
Bone loss;
Correlation
- From:
Chinese Journal of Immunology
2014;(7):870-873,878
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To demonstrate the relationship between the Th 1/Th2/Th17/Treg paradigm and the bone loss induced by estrogen deficiency and looking for potential target for clinical treatment.Methods:30 BALB/c mice were divided randomly into the normal control group , the sham operation group , and the ovariectomy group.The serum estradiol ( E2 ) was assessed by ELISA.Bone mineral density (BMD) of thigh bone was measured with dual energy X ray absorptiometry.Meanwhile,the T-cell subsets (Th1:CD4+TNFα+, Th2: CD4+IL-4+, Th17: CD4+IL-17 A+, Treg: CD4+CD25+Foxp3+) in spleen lymphocytes were analyzed by flow cytometry.Results:Compared with the normal group and the sham operation group , both E2 and BMD in the ovariectomy group decreased significantly ( P<0.05 ).The percentage of Th 1 and Th17 subset increased while the percentage of Th 2 and Treg decreased significantly in ovariectomy mice compared with sham operation mice.Correlation analysis showed that BMD was positively related to E 2 level and the percentage of Th 2 and Treg subset;however ,BMD was negatively related to the percentage of Th 1 and Th17 subset ( P<0.05 ).Conclusion: Conclusion: T-cell paradigm was involved in the bone loss induced by estrogen deficiency.Modifying T-cell paradigm may become a potential target for reducing bone loss induced by estrogen deficiency .
