Effects of different types of music on neuronal apoptosis of hippocampus of brain tissue of aged rats
10.3969/j.issn.1000-484X.2014.07.008
- VernacularTitle:不同类型音乐对老年大鼠脑组织海马区神经细胞凋亡的影响
- Author:
Ying HE
;
Shumei LI
;
Hongxi MA
- Publication Type:Journal Article
- Keywords:
Music;
Brain tissue;
Caspase-3;
Livin;
ELISA;
Immunohistochemistry;
Real-time PCR
- From:
Chinese Journal of Immunology
2014;(7):898-900,908
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of different types of music on Caspase-3 and Livin expression levels of the hippocampus of brain tissue of aged rats.Methods: Healthly elderly male Wistar rats 40 were randomly divided into :control group , Mozart music group ,Liangzhu music group and rock music group ,n=10;water maze training every morning while music intervention 2 h,afternoon pure music intervention 2 h,continuous 7 d,the eighth days ,the rats were sacrificed and taking blood and brain tissue ,by ELISA in serum and brain tissue Caspase-3 and Livin protein contents change;using immunohistochemistry assay in rat brain tissue Caspase-3 and Livin protein levels;real-time PCR detection of rat brain tissue changes in gene level of Caspase-3 and Livin.Results:Compared with the control group ,Mozart music group ,Liangzhu music rats the protein content ,protein level and gene level of Caspase-3 of serum and brain tissue were lower statistically significant ( P<0.01 ) , Livin was increased there was statistically significant ( P<0.01 ) ,Mozart music group and Liangzhu music group were no significant difference ( P>0.05 );while rock music group the protein content,protein level and gene level of Caspase-3 and Livin of brain tissue were not significantly difference (P>0.05).Conclusion:Mozart music group and Liangzhu music group by reducing serum and brain tissue Caspase -3 and increased Livin , to inhibit the apoptosis of the body′s nerve cells,which play a neuroprotective effect;the rock music group in the present study ineffective.
