Experimental study on in vitro preparation of adoptive immunotherapy cells induced by CD40 in combination with cytokines
10.3969/j.issn.1000-484X.2014.07.015
- VernacularTitle:CD40信号联合细胞因子体外制备过继免疫治疗细胞的实验研究
- Author:
Tengfei GAO
;
Yanzheng GU
;
Junchi XU
;
Yu SHEN
;
Xiaochen LI
;
Yibei ZHU
;
Xueguang ZHANG
- Publication Type:Journal Article
- Keywords:
Adoptive immunotherapy cell;
CD40-agonist monoclonal antibody 5C11;
IFN-α;
IL-7
- From:
Chinese Journal of Immunology
2014;(7):933-936,941
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore a new method of the cultivation of adoptive immunotherapy cells.Methods: Mononuclear cells was isoplated by density gradient centrifugation and then proliferated by using CD 40-agonist monoclonal antibody 5C11、cytokine of IFN-αand IL-7(CD40 group)in vitro.During the culturing procedure ,the cell morphology was obersved by optical microscope.The percentage of T-lymphocytes, NK-T cells, Treg cells and the cell proliferation, which were compared with CIK group CD3mAb activated,was detected on the 9th day.Results:There was no significant difference of CD 4+/CD8+T cells percentage between the two groups.But the Treg cells percentage of CIK group was far higher than that of CD 40 group,while the percentage of CD3+CD56+NK-T cells was lower than that of CD 40 group.And a group of Mo-NK-DC cells were observed in the CD 40 group.Conclusion: The new method of adoptive immunity therapy has been established in this study could increase the percentage of NK -T cells which had the ability to kill tumor cells.Simultaneously ,it is reduced the amount of Treg cells significantly.
