Effects of Qishu Granule on PI3K/Akt Signaling Transduction Pathways in the Process of Hepatic Fibrosis
10.3969/j.issn.1005-5304.2014.07.017
- VernacularTitle:芪术颗粒对肝纤维化形成过程中磷脂酰肌醇-3-激酶/丝氨酸蛋白激酶信号传导通路的影响
- Author:
Shaoneng LIU
;
Qinggao YANG
;
Peng PAN
;
Xiaping TAO
;
Huimin LIU
- Publication Type:Journal Article
- Keywords:
Qishu granule;
PI3K/Akt;
signaling transduction pathway;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(7):58-60,61
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Qishu granule on PI3K/Akt signaling transduction pathways in the process of hepatic fibrosis, and further explain the anti-hepatic fibrosis mechanism of Qishu granule.Methods Wistar rats were randomly divided into normal group, model group, experimental control group and Qishu granule group. Liver fibrosis was duplicated in rats by intraperitoneal injection of CCl4, and the rats were given appropriate treatment at the same day. Rats in Qishu granule group were given a gavage 2 g/(kg?d), 1.0 mL/100 g, while rats in experimental control group and normal group were given the same amount of aquae sterilisata. Rats in each group were taken liver tissue samples in the 1st, 2nd and 4th week, and were checked for the protein expression levels of p-Akt (Ser473), p-Akt (Thr308), Bad (Ser136) and Caspase9 by Western blot.Results Compared with the model group, expression levels of p-Akt (Ser473), p-Akt (Thr308), Bad (Ser136) and Caspase9 in model and Qishu granule groups increased in every time points (P<0.05). Compared with the model group, expression level of p-Akt (Ser473) in Qishu granule group decreased significantly in the 1st, 2nd, and 4th weeks, expression level of Caspase9 dropped in the 1st and the 4th weeks, with statistical significance (P<0.05,P<0.01);expressions of p-Akt (Thr308) and Bad (Ser136) were lower than those in model group, without statistical significance (P>0.05).Conclusion Qishu granule could regulate PI3K/Akt signaling transduction pathways, and inhibit the occurrence and development of liver fibrosis.
